In sickle-cell anemia, for example, the exact nucleotide change in the mutant gene is known (the sequence GAG is changed to GTG at a specific point in the DNA strand that codes for the β chain of hemoglobin). One goal of modern medicine is to identify those fetuses that carry two copies of the defective gene long before birth so that the mother, if she wishes, can have the pregnancy terminated. In most of these the mutation is recessive: that is, it shows its effect only when an individual inherits two defective copies of the gene, one from each parent. More than 3000 human genetic diseases are attributable to single-gene defects.Comparison of Southern, Northern, and Western blotting techniques.Analysis of protein samples by SDS polyacrylamide-gel electrophoresis and Western blotting Protein bands detected by specific antibody SDS-PAGE Western blot.SDS polyacrylamide-gel electrophoresis (SDS-PAGE).Western blotting, or immunoblotting Technique for detecting specific proteins separated by electrophoresis by use of labeled antibodies.An example of Northern blotting Northern blot RNA gel 28 S 18 S.Lower background Higher background Cannot be exposed to basic solution Can be exposed to basic solution Not easily reprobed Can be reprobed several times Comparison of nitrocellulose and nylon membranes NC Nylon Hydrophobic binding Covalent binding Fragile Durable Probe length > 200 ~ 300 bp PCR Labeling, Random Primed Labeling, and RNA Labeling.Detection of the sickle-cell globin gene by Southern blotting.Detection of an RFLP by Southern blotting.Comparison of Southern, Northern, and Western analyses of Gene X.Southern, Northern and Western blotting.d) detect the presence of a particular protein molecule in Western blottingħ. Multiple Choice Question on DNA fingerprintingĤ.Multiple Choice Question on DNA extraction.Multiple Choice Question on Molecular Biology Techniques.DNA fingerprinting-D)antigen antibody reaction Aminobenzyloxymethyl filter paper is commonly used for transfer inĭ) Dot blotting 10. Which of the following technique is most suitable for detecting the presence of a gene productĭ) Western blotting 9. Which of the following technique doesn’t involve electrophoresis for the separation of biomoleculesĪ) protein for detecting a specific DNA moleculeī) short piece of labelled DNA which are complementary to the nucleic acid strand to be detectedĬ) short piece of labelled DNA or RNA which are complementary to the nucleic acid strand to be detectedĭ) none of these 8. Which of the following technique is suitable for identifying mRNA molecule in a sampleĭ) Eastern blotting 6. Labelled Antibodies are used to detectĪ) detect the presence of a particular DNA molecule in Southern blottingī) detect the presence of a particular RNA molecule in Southern blottingĬ) detect the presence of a particular protein molecule in Southern blottingĭ) detect the presence of a particular protein molecule in Western blotting 5. Arrange the following in correct orderĭ) 1-B, 2-C, 3-A, 4-C 4. Which of the following technique is used in DNA finger printing?ĭ) Eastern blotting 3.
Western blotting is the technique for the detection ofĭ) specific glycolipid in a sample 2.
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